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Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed <t>under</t> <t>blue/green</t> <t>LED</t> <t>light.</t> Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.
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Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed <t>under</t> <t>blue/green</t> <t>LED</t> <t>light.</t> Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.
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Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed <t>under</t> <t>blue/green</t> <t>LED</t> <t>light.</t> Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.
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Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed <t>under</t> <t>blue/green</t> <t>LED</t> <t>light.</t> Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.
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Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed under blue/green LED light. Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.

Journal: Scientific Reports

Article Title: Development of a global screening system for detecting protein–protein interactions by luminescence complementation in fission yeast

doi: 10.1038/s41598-026-35430-8

Figure Lengend Snippet: Correlation between genomic mCherry copy number and fluorescence intensity. (Top) Scatter plot showing the relationship between the relative genomic copy number of the mCherry gene and its fluorescence intensity. The mCherry copy number was normalized to that of the endogenous act1 gene using quantitative real-time PCR. Fluorescence intensity was normalized to cell density (OD₆₀₀). Each data point represents an independent transformant generated by pPREY3 integration. The plot reveals a positive correlation between normalized mCherry copy number ( mCherry / act1 ) and normalized fluorescence intensity (mCherry fluorescence/OD₆₀₀), indicating that observed fluorescence reflects integrated mCherry copy number. A trendline is indicated by a dashed line. (Bottom) mCherry fluorescence of pPREY3 integrants used for qPCR, observed under blue/green LED light. Cells at positions C10-12 correspond to the host strains lacking the mCherry expression unit.

Article Snippet: The Leu + transformants obtained by the introduction of the prey library were exposed to blue/green LED light (FAS-V, NIPPON Genetics).

Techniques: Fluorescence, Real-time Polymerase Chain Reaction, Generated, Expressing